Sweet corn hybrid shy6rh1365 and parents thereof

ABSTRACT

The invention provides seed and plants of sweet corn hybrid SHY6RH1365 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of sweet corn hybrid SHY6RH1365 and the parent lines thereof, and to methods for producing a sweet corn plant produced by crossing such plants with themselves or with another sweet corn plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the parts of such plants.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, morespecifically, to the development of sweet corn hybrid SHY6RH1365 and theinbred sweet corn lines SHY-6RLAL201 and SHY 084-5055.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits ina single variety/hybrid. Such desirable traits may include any traitdeemed beneficial by a grower and/or consumer, including greater yield,better stalks, better roots, resistance to insecticides, herbicides,pests, and disease, tolerance to heat and drought, reduced time to cropmaturity, better agronomic quality, higher nutritional value, sugarcontent, uniformity in germination times, stand establishment, growthrate and maturity, among others.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant or plant variety. A plant cross-pollinates if pollencomes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants of different genotypes produces auniform population of hybrid plants that are heterozygous for many geneloci. Conversely, a cross of two plants each heterozygous at a number ofloci produces a population of hybrid plants that differ genetically andare not uniform. The resulting non-uniformity makes performanceunpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants, and theevaluation of the crosses. Pedigree breeding and recurrent selection areexamples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines and hybrids derivedtherefrom are developed by selfing and selection of desired phenotypes.The new lines and hybrids are evaluated to determine which of those havecommercial potential.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides a plant of the sweet cornhybrid designated SHY6RH1365, the sweet corn line SHY-6RLAL201 or sweetcorn line SHY 084-5055. Also provided are corn plants having all thephysiological and morphological characteristics of such a plant. Partsof these corn plants are also provided, for example, including pollen,an ovule, and a cell of the plant.

In another aspect of the invention, a plant of sweet corn hybridSHY6RH1365 and/or sweet corn lines SHY-6RLAL201 and SHY 084-5055comprising an added heritable trait is provided. The heritable trait maycomprise a genetic locus that is, for example, a dominant or recessiveallele. In one embodiment of the invention, a plant of sweet corn hybridSHY6RH1365 and/or sweet corn lines SHY-6RLAL201 and SHY 084-5055 isdefined as comprising a single locus conversion. In specific embodimentsof the invention, an added genetic locus confers one or more traits suchas, for example, male sterility, herbicide resistance, insectresistance, resistance to bacterial, fungal, sugar content, nematode orviral disease, and altered fatty acid, phytate or carbohydratemetabolism. In further embodiments, the trait may be conferred by anaturally occurring gene introduced into the genome of a line bybackcrossing, a natural or induced mutation, or a transgene introducedthrough genetic transformation techniques into the plant or a progenitorof any previous generation thereof. When introduced throughtransformation, a genetic locus may comprise one or more genesintegrated at a single chromosomal location.

The invention also concerns the seed of sweet corn hybrid SHY6RH1365and/or sweet corn lines SHY-6RLAL201 and SHY 084-5055. The corn seed ofthe invention may be provided, in one embodiment of the invention, as anessentially homogeneous population of corn seed of sweet corn hybridSHY6RH1365 and/or sweet corn lines SHY-6RLAL201 and SHY 084-5055.Essentially homogeneous populations of seed are generally free fromsubstantial numbers of other seed. Therefore, seed of hybrid SHY6RH1365and/or sweet corn lines SHY-6RLAL201 and SHY 084-5055 may, in particularembodiments of the invention, be provided forming at least about 97% ofthe total seed, including at least about 98%, 99% or more of the seed.The seed population may be separately grown to provide an essentiallyhomogeneous population of sweet corn plants designated SHY6RH1365 and/orsweet corn lines SHY-6RLAL201 and SHY 084-5055.

In yet another aspect of the invention, a tissue culture of regenerablecells of a sweet corn plant of hybrid SHY6RH1365 and/or sweet corn linesSHY-6RLAL201 and SHY 084-5055 is provided. The tissue culture willpreferably be capable of regenerating corn plants capable of expressingall of the physiological and morphological characteristics of thestarting plant, and of regenerating plants having substantially the samegenotype as the starting plant. Examples of some of the physiologicaland morphological characteristics of the hybrid SHY6RH1365 and/or sweetcorn lines SHY-6RLAL201 and SHY 084-5055 include those traits set forthin the tables herein. The regenerable cells in such tissue cultures maybe derived, for example, from embryos, meristematic cells, immaturetassels, microspores, pollen, leaves, anthers, roots, root tips, silk,flowers, kernels, ears, cobs, husks, or stalks, or from callus orprotoplasts derived from those tissues. Still further, the presentinvention provides corn plants regenerated from a tissue culture of theinvention, the plants having all the physiological and morphologicalcharacteristics of hybrid SHY6RH1365 and/or sweet corn linesSHY-6RLAL201 and SHY 084-5055.

In still yet another aspect of the invention, processes are provided forproducing corn seeds, plants and parts thereof, which processesgenerally comprise crossing a first parent corn plant with a secondparent corn plant, wherein at least one of the first or second parentcorn plants is a plant of sweet corn line SHY-6RLAL201 or sweet cornline SHY 084-5055. These processes may be further exemplified asprocesses for preparing hybrid corn seed or plants, wherein a first cornplant is crossed with a second corn plant of a different, distinctgenotype to provide a hybrid that has, as one of its parents, a plant ofsweet corn line SHY-6RLAL201 or sweet corn line SHY 084-5055. In theseprocesses, crossing will result in the production of seed. The seedproduction occurs regardless of whether the seed is collected or not.

In one embodiment of the invention, the first step in “crossing”comprises planting seeds of a first and second parent corn plant, oftenin proximity so that pollination will occur for example, naturally ormanually. Where the plant is self-pollinated, pollination may occurwithout the need for direct human intervention other than plantcultivation. For hybrid crosses, it may be beneficial to detassel orotherwise emasculate the parent used as a female.

A second step may comprise cultivating or growing the seeds of first andsecond parent corn plants into mature plants. A third step may comprisepreventing self-pollination of the plants, such as by detasseling orother means.

A fourth step for a hybrid cross may comprise cross-pollination betweenthe first and second parent corn plants. Yet another step comprisesharvesting the seeds from at least one of the parent corn plants. Theharvested seed can be grown to produce a corn plant or hybrid cornplant.

The present invention also provides the corn seeds and plants producedby a process that comprises crossing a first parent corn plant with asecond parent corn plant, wherein at least one of the first or secondparent corn plants is a plant of sweet corn hybrid SHY6RH1365 and/orsweet corn lines SHY-6RLAL201 and SHY 084-5055. In one embodiment of theinvention, corn seed and plants produced by the process are firstgeneration (F₁) hybrid corn seed and plants produced by crossing a plantin accordance with the invention with another, distinct plant. Thepresent invention further contemplates plant parts of such an F₁ hybridcorn plant, and methods of use thereof. Therefore, certain exemplaryembodiments of the invention provide an F₁ hybrid corn plant and seedthereof.

In still yet another aspect, the present invention provides a method ofproducing a plant derived from hybrid SHY6RH1365 and/or sweet corn linesSHY-6RLAL201 and SHY 084-5055, the method comprising the steps of: (a)preparing a progeny plant derived from hybrid SHY6RH1365 and/or sweetcorn lines SHY-6RLAL201 and SHY 084-5055, wherein said preparingcomprises crossing a plant of the hybrid SHY6RH1365 and/or sweet cornlines SHY-6RLAL201 and SHY 084-5055 with a second plant; and (b)crossing the progeny plant with itself or a second plant to produce aseed of a progeny plant of a subsequent generation. In furtherembodiments, the method may additionally comprise: (c) growing a progenyplant of a subsequent generation from said seed of a progeny plant of asubsequent generation and crossing the progeny plant of a subsequentgeneration with itself or a second plant; and repeating the steps for anadditional 3-10 generations to produce a plant derived from hybridSHY6RH1365 and/or sweet corn lines SHY-6RLAL201 and SHY 084-5055. Theplant derived from hybrid SHY6RH1365 and/or sweet corn linesSHY-6RLAL201 and SHY 084-5055 may be an inbred line, and theaforementioned repeated crossing steps may be defined as comprisingsufficient inbreeding to produce the inbred line. In the method, it maybe desirable to select particular plants resulting from step (c) forcontinued crossing according to steps (b) and (c). By selecting plantshaving one or more desirable traits, a plant derived from hybridSHY6RH1365 and/or sweet corn lines SHY-6RLAL201 and SHY 084-5055 isobtained which possesses some of the desirable traits of the line/hybridas well as potentially other selected traits.

In certain embodiments, the present invention provides a method ofproducing food or feed comprising: (a) obtaining a plant of sweet cornhybrid SHY6RH1365 and/or sweet corn lines SHY-6RLAL201 and SHY 084-5055,wherein the plant has been cultivated to maturity, and (b) collecting atleast one corn from the plant.

In still yet another aspect of the invention, the genetic complement ofsweet corn hybrid SHY6RH1365 and/or sweet corn lines SHY-6RLAL201 andSHY 084-5055 is provided. The phrase “genetic complement” is used torefer to the aggregate of nucleotide sequences, the expression of whichsequences defines the phenotype of, in the present case, a sweet cornplant, or a cell or tissue of that plant. A genetic complement thusrepresents the genetic makeup of a cell, tissue or plant, and a hybridgenetic complement represents the genetic make up of a hybrid cell,tissue or plant. The invention thus provides corn plant cells that havea genetic complement in accordance with the corn plant cells disclosedherein, and seeds and plants containing such cells.

Plant genetic complements may be assessed by genetic marker profiles,and by the expression of phenotypic traits that are characteristic ofthe expression of the genetic complement, e.g., isozyme typing profiles.It is understood that hybrid SHY6RH1365 and/or sweet corn linesSHY-6RLAL201 and SHY 084-5055 could be identified by any of the manywell known techniques such as, for example, Simple Sequence LengthPolymorphisms (SSLPs) (Williams et al., Nucleic Acids Res., 18:6531-6535, 1990), Randomly Amplified Polymorphic DNAs (RAPDs), DNAAmplification Fingerprinting (DAF), Sequence Characterized AmplifiedRegions (SCARs), Arbitrary Primed Polymerase Chain Reaction (AP-PCR),Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858,specifically incorporated herein by reference in its entirety), andSingle Nucleotide Polymorphisms (SNPs) (Wang et al., Science,280:1077-1082, 1998).

In still yet another aspect, the present invention provides hybridgenetic complements, as represented by corn plant cells, tissues,plants, and seeds, formed by the combination of a haploid geneticcomplement of a corn plant of the invention with a haploid geneticcomplement of a second corn plant, preferably, another, distinct cornplant. In another aspect, the present invention provides a corn plantregenerated from a tissue culture that comprises a hybrid geneticcomplement of this invention.

In still yet another aspect, the invention provides a method ofdetermining the genotype of a plant of sweet corn hybrid SHY6RH1365and/or sweet corn lines SHY-6RLAL201 and SHY 084-5055 comprisingdetecting in the genome of the plant at least a first polymorphism. Themethod may, in certain embodiments, comprise detecting a plurality ofpolymorphisms in the genome of the plant. The method may furthercomprise storing the results of the step of detecting the plurality ofpolymorphisms on a computer readable medium. The invention furtherprovides a computer readable medium produced by such a method.

Any embodiment discussed herein with respect to one aspect of theinvention applies to other aspects of the invention as well, unlessspecifically noted.

The term “about” is used to indicate that a value includes the standarddeviation of the mean for the device or method being employed todetermine the value. The use of the term “or” in the claims is used tomean “and/or” unless explicitly indicated to refer to alternatives onlyor the alternatives are mutually exclusive. When used in conjunctionwith the word “comprising” or other open language in the claims, thewords “a” and “an” denote “one or more,” unless specifically notedotherwise. The terms “comprise,” “have” and “include” are open-endedlinking verbs. Any forms or tenses of one or more of these verbs, suchas “comprises,” “comprising,” “has,” “having,” “includes” and“including,” are also open-ended. For example, any method that“comprises,” “has” or “includes” one or more steps is not limited topossessing only those one or more steps and also covers other unlistedsteps. Similarly, any plant that “comprises,” “has” or “includes” one ormore traits is not limited to possessing only those one or more traitsand covers other unlisted traits.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and any specificexamples provided, while indicating specific embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants,seeds and derivatives of sweet corn hybrid SHY6RH1365, sweet corn lineSHY-6RLAL201 and sweet corn line SHY 084-5055. The hybrid SHY6RH1365 wasproduced by the cross of parent lines SHY-6RLAL201 and SHY 084-5055. Theparent lines show uniformity and stability within the limits ofenvironmental influence. By crossing the parent lines, uniform seedhybrid SHY6RH1365 can be obtained.

Hybrid SHY6RH1365 is a yellow sh2 sweet corn. Hybrid SHY6RH1365 has theRpG gene which provides resistance to some races of Puccinia sorghi(common rust) (Hu, G. and S. H. Hulbert. 1996. Construction of‘compound’ rust genes in maize. Euphytica 87: 45-51).

SHY-6RLAL201 is a yellow sweet corn homozygous for the recessive genesh2. Line SHY-6RLAL201 has the RpG gene which provides resistance tosome races of Puccinia sorghi (common rust) (Hu, G. and S. H. Hulbert.1996. Construction of ‘compound’ rust genes in maize. Euphytica 87:45-51). SHY-6RLAL201 resembles inbred lines SYY093-678, SYY093-7001, andSYY-6R07003.

The development of sweet corn hybrid SHY6RH1365 and its parent lines issummarized below.

A. ORIGIN AND BREEDING HISTORY OF SWEET CORN HYBRID SHY6RH1365

The hybrid SHY6RH1365 was produced by the cross of parent linesSHY-6RLAL201 and SHY 084-5055.

SHY-6RLAL201 is a yellow sweet corn inbred which is homozygous for therecessive sh2 gene. The line also has the RpG gene which providesresistance to some races of Puccinia sorghi (Hu, G. and S. H. Hulbert.1996. Construction of ‘compound’ rust genes in maize. Euphytica 87:45-51).

SHY-6RLAL201 was produced by breeding and selection as described below:

Parent Line 1:

-   -   Winter, Year 1-Year 2: The inbred line CODE6-5 (a proprietary        Seminis inbred) was grown in a Hawaii nursery on Molokai and was        crossed onto a stock carrying the RpG allele. This stock was        obtained from Dr. Jerald Pataky of the University of Illinois.        It was coded Seminis accession B142 and was an R168 field corn        inbred converted to carry the RpG allele. This allele was        reported by Dr. Art Hooker to have come from PI 163558. CODE6-5        was grown in Hawaii nursery row 9790 and B142 was grown in        nursery row 9764. The nursery was grown by Hawaiian Research        Ltd. under a contract with Seminis. An ear harvested of this F1        cross was given a source of H00: 9790×9764/2.    -   Summer, Year 2: Seeds of the F1 with source H00: 9790×9764/2        were planted in the Seminis DeForest, Wis. nursery row 4188 and        were crossed with inbred line CODE6-5 (a proprietary Seminis        inbred) grown in row 4187. This made a BC1 generation. An ear        harvested of this BC1 cross was given the source of N00:        4188×4187/1.    -   Winter, Year 2-Year 3: Seeds of the BC1 with source N00:        4188×4187/1 were planted in the Seminis Melipilla, Chile nursery        row 7584 and were crossed with inbred line CODE6-5 (a        proprietary Seminis inbred) grown in row 7583. This made a BC2        generation. An ear harvested of this BC1 cross was given the        source of E01: 7584×7583/1.    -   Winter, Year 3-Year 4: Seeds of the BC2 with source E01:        7584×7583/1 were planted in Homestead, Fla. nursery row 513 and        was cross pollinated by SYY093-678 (a proprietary Seminis        inbred) in nursery row 512 to make an F1. The nursery was grown        for Seminis by 27 Farms who were paid for their services. One of        the ears of the F1 cross was given a source of C02: 513×512/2.    -   Summer, Year 4: Seeds of the F1 with source C02: 513×512/2 were        planted in Seminis DeForest, Wis. nursery row 5219 and cross        pollinated by SYY093-678 (a proprietary Seminis inbred) in        nursery row 5218 to make a BC1. One of the ears of the BC1 cross        was give a source of N02: 5219×5218/1.    -   Winter, Year 4-Year 5: Seeds of the BC1 with source N02:        5219×5218/1 were planted in the Seminis Melipilla, Chile nursery        row 8956 and cross pollinated by SYY093-678 (a proprietary        Seminis inbred) grown in nursery row 8955 to make a BC2. One of        the ears of the BC2 cross was give the source of E03:        8956×8955/2.    -   Summer, Year 5: Seeds of the BC2 with source E03: 8956×8955/2        were planted in the Seminis DeForest, Wis. nursery row 5863.        Plants were inoculated with Puccinia sorghi and some of the        resistant plants were cross pollinated by SYY093-678 (a        proprietary Seminis inbred) grown in nursery row 5858 to make a        BC3. One of the ears of the BC3 cross was give the source of        N03: 5863×5858/1.    -   Winter, Year 5-Year 6: Seeds of the BC3 with source N03:        5863×5858/1 were planted in the Seminis Melipilla, Chile nursery        row 5018 and self pollinated to make a BC3F2. One of the        harvested BC3F2 ears was given the source of E04: 5018/1. and        the name designation of N2785.    -   Summer, Year 6: Seeds of the BC3F2 with source E04: 5018/1* were        planted in the Seminis DeForest, Wis. nursery row 4037. Plants        were inoculated with Puccinia sorghi and some of the common rust        resistant plants were self pollinated to make the BC3F3        generation. N04: 4037/2. was the source designation given to one        of the selfed ears following harvest.    -   Winter, Year 7: Seeds of the BC3F3 with source N04: 4037/2. were        planted in the Seminis Melipilla, Chile nursery row 8525 and        self pollinated to make a BC3F4. E05: 8525/1. was the source        given to one of the BC3F4 ears.

Parent Line 2:

-   -   Winter, Year 3-Year 4: Inbred N1217NP2 (a proprietary Seminis        inbred now named SHW084-5006) was grown in the Seminis        Melipilla, Chile nursery in row 5437. This line was crossed by        inbred SYY093-678 grown in row 5427. The F1 cross was given a        hybrid designation of SVR 08717186. E02: 5437×5427 was the        source designation given to the cross.    -   Winter, Year 4-Year 5: Hybrid SVR08717186 from source E02:        5437×5427 was grown in the Seminis Melipilla, Chile nursery in        row 9212. This line was crossed by inbred SYY093-678 grown in        row 9205 grown from source 208796 to make the BC1. The BC1 cross        was given the source designation E03: 9212×9205 and the name        N1999.    -   Summer, Year 5: Seeds of the BC1 from source E03: 9212×9205 were        planted in the Seminis DeForest, Wis. nursery row 6626 and        plants were self pollinated to make the BC1F2. N03: 6626/1. was        the source given to one of the self pollinated ears which was        segregating for Y1 (yellow and white kernels).    -   Winter, Year 5-Year 6: White kernels of the BC1F2 from source        N03: 6626/1. were planted in the Seminis Melipilla, Chile        nursery in row 5793. and plants were self pollinated to make the        BC1F3. E04: 5793/1. Was the source given to one of the self        pollinated ears. All kernels were white.    -   Summer, Year 6: Seeds of the BC1F3 from source E04: 5793/1. were        planted in the Seminis DeForest, Wis. nursery row 5585 and        plants were self pollinated to make the BC1F4. N04: 5585/1. was        the source given to one of the self pollinated ears. It was        noted that the kernels were homozygous sh2 but did not have the        su1 gene based on kernel phenotype.    -   Winter, Year 6-Year 7: Seeds of the BC1F4 from source N04:        5585/1. were planted in the Seminis Homestead, Fla. nursery row        374 and self pollinated to make the BC1F5. The nursery was grown        for Seminis by 27 Farms who were paid for their services. C05:        374/1. was the source given to one of the self pollinated ears.    -   Summer, Year 7: Seeds of the BC1F5 from source C05: 374/1. were        planted in the Seminis DeForest, Wis. nursery in row 6912 and        plants were crossed by inbred SYY093-678 grown in row 6911. N05:        6912×6911/4 was the source given to one of the harvested ears        from this cross.    -   Winter, Year 7-Year 8: Seeds of the parent I line from source        E05: 8525/1. were grown in the Seminis Melipilla, Chile nursery        in range 20 column 8. Plants were crossed by parent 2 from        source N05: 6912×6911/4 grown in range 20 column 29. 05 10 6R 6R        MSME-E2_(—)00020_(—)00028_@_/ was the source given to a bulk of        pollinated ears from this cross. This made the F1 generation.    -   Summer, Year 8: Seeds from source 05 10 6R 6R        MSME-E2_(—)00020_(—)00028_@_/ were grown in range 123 column 22        of the Monsanto Kihei, Hi. nursery field HIKIK40-KHI-KAU1-31.        Plants were cross pollinated by a haploid inducer stock.        Harvested kernels of the DH0 generation were given the source        designation 06 06 31 31        HIKIK40-KHI-KAU1-31_(—)00123_(—)00022_@_%.    -   Winter, Year 8-Year 9: Putative haploid kernels were selected        from source 06 06 31 31        HIKIK40-KHI-KAU1-31_(—)00123_(—)00022_@_%. These kernels were        treated with colchicine during germination and the germinated        seedlings were transplanted to the Seminis Melipilla, Chile        nursery. Plants which shed pollen were self pollinated to make        the DH1 generation. Kernels harvested are expected to be doubled        haploids. The source designation 06 10 6R 6R        MSME-EDH_(—)00001_(—)00010_(—)2_. was given to one of the        harvested doubled haploid ears. This line was given the name        SHY-6RLAL201.    -   SUMMER, Year 9: Seed of inbred SHY-6RLAL201 from source 06 10 6R        6R MSME-EDH_(—)00001_(—)00010_(—)2_. was planted in range 6        column 23 of field WIDE-IB1 of the Seminis DeForest, Wis.        station and plants were self pollinated to make the DH2        generation. 07 04 6R 6R WIDE-IB1_(—)00006_(—)00023_@_. was the        source given to a bulk of the harvested self pollinated ears.    -   Winter, Year 9-Year 10: Seed of source 07 04 6R 6R        WIDE-IB1_(—)00006_(—)00023_@_. was planted in range 8 column 44        of field MSME-E2 of the Seminis Melipilla, Chile nursery. Plants        were self pollinated to make the DH3 generation. 07 10 6R 6R        MSME-E2_(—)00008_(—)00044_(—)1_. was the source given to one of        the harvested ears.    -   Winter, Year 10-Year 11: Seed of source 07 10 6R 6R        MSME-E2_(—)00008_(—)00044_(—)1_. was grown in the Seminis        Melipilla, Chile nursery in range 10 column 1 of field MSME-E1        and plants were self pollinated to make the DH4 generation. 08        10 6R 6R MSME-E1_(—)00010_(—)00001_(—)1_. was the source given        to one of the self pollinated ears.    -   Winter, Year 11-Year 12: Seed from source 08 10 6R 6R        MSME-E1_(—)00010_(—)00001_(—)1_. was planted in range 28 columns        6-13 of field MSME-E1 in the Seminis Melipilla, Chile nursery        and plants were self pollinated to make the DH5 generation.        Plants and harvested ears were observed to be uniform for all        traits observed. Twenty-seven of the harvested ears from these        rows were given the source designation 09 10 6R 6R        MSME-E1_(—)00028_(—)00013_(—)1_. thru 09 10 6R 6R        MSME-E1_(—)00028_(—)00013_(—)27_. and these ears were sent to        Foundation Seed as the breeder's seed increase of this inbred.        -   In winter Year 12, 30 plants from source 08 10 6R 6R            MSME-E1_(—)00010_(—)00001_(—)1_. were grown in the            greenhouse and inoculated with a d-virulent isolate of            Puccinia sorghi. All 30 plants were resistant which verified            the presence of the RpG gene in this stock and indicates the            line is homozygous for this allele.

Sweet corn inbred SHY-6RLAL201 was reproduced by self pollination in thewinter Year 11-Year 12 in Melipilla, Chile nursery and was judged to bestable. 27 single ears were sent to Foundation Seed as Breeder's Seed.Inbred SHY-6RLAL201 is uniform for all traits observed. SHY-6RLAL201shows no variants other than what would normally be expected due toenvironment or that would occur for almost any character during thecourse of repeated sexual reproduction.

Sweet corn line SHY 084-5055 originated from a RXY 8301×HXP 3365 cross.

B. PHYSIOLOGICAL AND MORPHOLOGICAL CHARACTERISTICS OF SWEET CORN HYBRIDSHY6RH1365, SWEET CORN LINE SHY-6RLAL201 AND SWEET CORN LINE SHY084-5055

In accordance with one aspect of the present invention, there isprovided a plant having the physiological and morphologicalcharacteristics of sweet corn hybrid SHY6RH1365 and the parent linesthereof. A description of the physiological and morphologicalcharacteristics of such plants is presented in Tables 1-3.

TABLE 1 Physiological and Morphological Characteristics of HybridSHY6RH1365 Comparison Variety - Characteristic SHY6RH1365 Basin R 1.Type sweet sweet 2. Maturity in the Region of Best Adaptability fromemergence to 50% of days: 49 days: 49 plants in silk heat units: 977.3heat units: 956.4 from emergence to 50% of days: 49 days: 47 plants inpollen heat units: 939 heat units: 925.4 from 10% to 90% pollen sheddays: 4 days: 5 heat units: 88.3 heat units: 88.3 from 50% silk tooptimum days: 23 days: 20 edible quality heat units: 409.7 heat units:402.3 from 50% silk to harvest at days: 53 days: 55 25% moisture heatunits: 1092.9 heat units: 1110.3 foliage: intensity of green dark darkcolor 3. Plant length (tassel included) medium long (inbred lines only)ratio height of insertion of large small upper ear to plant lengthpeduncle length medium medium plant height (to tassel tip) 185.6 cm214.2 cm standard deviation: standard deviation 10.5817 7.47 samplesize: 15 sample size: 15 ear height (to base of top ear 65.4 cm 70.2 cmnode) standard deviation: standard deviation 9.0089 6.13 sample size: 15sample size: 15 length of top ear internode 16.2 cm 15.3 cm standarddeviation: standard deviation: 1.1159 1.94 sample size: 15 sample size:15 average number of tillers 2.6 avg 1.2 avg standard deviation:standard deviation 0.8281 0.41 sample size: 15 sample size: 15 averagenumber of ears per 1.6 avg 1.6 avg stalk standard deviation: standarddeviation: 0.488 0.51 sample size: 15 sample size: 15 anthocyanin ofbrace roots absent absent 4. Leaf first leaf: anthocyanin absent or veryweak absent or very weak coloration of sheath first leaf: shape of apexpointed pointed undulation of margin of blade intermediate intermediateangle between blade and stem small (±25°) medium (±50°) (on leaf justabove upper ear) curvature of blade moderately recurved moderatelyrecurved anthocyanin coloration of absent or very weak absent or veryweak sheath (in middle of plant) width of blade medium medium width ofear node leaf in 9.5 cm 9.2 cm centimeters standard deviation: standarddeviation: 0.6 0.623 sample size: 15 sample size: 15 length of ear nodeleaf in 86.1 cm 84.3 cm centimeters standard deviation: standarddeviation: 2.05 3.0441 sample size: 15 sample size: 15 number of leavesabove top 6 6.2 ear standard deviation: standard deviation: 0.75 0.378sample size: 15 sample size: 15 degrees leaf angle 33° 55° color 5GY 3/47.5GY 4/4 sheath pubescence (1 = none to 6 9 9 = like peach fuzz)marginal waves (1 = none to 4 3 9 = many) longitudinal creases (1 = none1 1 to 9 = many) stem: degree of zig-zag absent or very slight absent orvery slight stem: anthocyanin coloration absent or very weak absent orvery weak of brace roots stem: anthocyanin coloration absent or veryweak absent or very weak of internodes 5. Tassel time of anthesis (onmiddle medium early to medium third of main axis, 50% of plants)anthocyanin coloration at base absent or very weak absent or very weakof glume (in middle third of main axis) anthocyanin coloration of absentor very weak absent or very weak glumes excluding base anthocyanincoloration of absent or very weak absent or very weak anthers (in middlethird of main axis) angle between main axis and very small (<5°) medium(±50°) lateral branches (in lower third of tassel) curvature of lateralbranches slightly recurved moderately recurved (in lower third oftassel) number of primary lateral many medium branches density ofspikelets medium medium length of main axis above very long very longlowest lateral branch (A-B) length of main axis above long very longhighest lateral branch (C-D) length of lateral branch medium very longnumber of primary lateral 29 23.5 branches standard deviation: standarddeviation: 2.61 2.952 samples size: 15 sample size: 15 branch angle fromcentral 33.8° 49.6° spike standard deviation: standard deviation: 5.06917.19 sample size: 15 sample size: 15 length (from top leaf collar to 38cm 41.7 cm tassel tip) standard deviation: standard deviation: 2.75722.81 sample size: 15 sample size: 15 pollen shed (0 = male sterile to 55 9 = heavy shed) anther color 2.5GY 6/6 5GY 7/6 glume color 5GY 5/6 5GY6/4 bar glumes (glume bands) absent absent 6. Ear (unhusked data) silkcolor 2.5GY 8/4 2.5GY 7/6 (3 days after emergence) (unhusked data) freshhusk 2.5GY 6/10 2.5GY 6/8 color (25 days after 50% silking) (unhuskeddata) dry husk 5Y 8/4 2.5GY 8/4 color (65 days after 50% silking)(unhusked data) position of upright upright ear at dry husk stage(unhusked data) husk 6 6 tightness (1 = very loose to 9 = very tight)(unhusked data) husk medium (<8 cm) medium (<8 cm) extension (atharvest) (husked ear data) ear length 18.8 cm 21.2 cm standarddeviation: standard deviation: 1.0281 1.18 sample size: 15 sample size:15 (husked ear data) ear diameter 46.5 mm 41.7 mm at mid-point standarddeviation: standard deviation: 1.5403 2.36 sample size: 15 sample size:15 (husked ear data) ear weight 103.8 gm 117.6 gm standard deviation:standard deviation: 13.747 19.91 sample size: 15 sample size: 15 (huskedear data) number of 19.5 15.7 kernel rows standard deviation: standarddeviation: 1.6847 1.67 sample size: 15 sample size: 15 (husked ear data)kernel rows distinct distinct (husked ear data) row slightly curvedstraight alignment (husked ear data) shank length 16.2 cm 15.7 cm incentimeters standard deviation: standard deviation: 2.2561 3.34 samplesize: 15 sample size: 15 (husked ear data) ear taper slight averagelength medium long diameter (in middle) medium medium shape cylindricalconico-cylindrical number of rows of grain many medium number of colorsof grains one one (only varieties with ear type of grain: sweet or waxy)grain: intensity of yellow dark medium color (only varieties with eartype of grain: sweet) grain: length (only varieties medium long with eartype of grain: sweet) grain: width (only varieties medium broad with eartype of grain: sweet) type of grain sweet sweet shrinkage of top ofgrain (only medium medium varieties with ear type of grain: sweet) colorof top of grain yellow orange yellow orange anthocyanin coloration ofabsent or very weak absent or very weak glumes of cob time of silkemergence (50% medium early to medium of plants) anthocyanin colorationof absent or very weak absent or very weak silks 7. Kernel (dried)length 11.3 mm 11.2 mm standard deviation: standard deviation: 0.62360.72 sample size: 15 sample size: 15 width 6 mm 6.9 mm standarddeviation: standard deviation: 0.7818 0.82 sample size: 15 sample size:15 thickness 4 mm 3.8 mm standard deviation: standard deviation 8.21520.51 sample size: 15 sample size: 15 % round kernels (shape grade) 0% 0%sample size: n/a sample size: n/a aleurone color pattern homozygoushomozygous hard endosperm color 7.5YR 7/10 7.5YR 7/10 endosperm typesweet (su1) sweet (su1) weight per 100 kernels 17.0 gm 11 gm (unsizedsample) sample size: n/a sample size: n/a 8. Cob diameter at mid-point28.9 mm 29.1 mm standard deviation: standard deviation: 1.1211 3.41sample size: 15 sample size: 15 color 5Y 8/4 2.5Y 8/4 9. AgronomicTraits stay green (at 65 days after 6 6 anthesis) (from 1 = worst to 9 =excellent) dropped ears 0% 0% % pre-anthesis brittle 0% 0% snapping %pre-anthesis root lodging 0% 0% post-anthesis root lodging 0% 0% *Theseare typical values. Values may vary due to environment. Other valuesthat are substantially equivalent are also within the scope of theinvention.

TABLE 2 Physiological and Morphological Characteristics of Sweet CornLine SHY- 6RLAL201 Comparison Variety - Characteristic SHY-6RLAL201FA32 1. Type sweet sweet 2. Region where developed in the north centralnorth central U.S.A. 3. Maturity in the Region of Best Adaptability fromemergence to 50% of days: 60 days: 76 plants in silk heat units: 1129.05heat units: 1351.92 from emergence to 50% of days: 59 days: 80 plants inpollen heat units: 1107.35 heat units: 1436.32 from 10% to 90% pollenshed days: 6 days: 9 heat units: 153.25 heat units: 177.35 from 50% silkto optimum days: 17 days: 20 edible quality heat units: 377.8 heatunits: 412.22 from 50% silk to harvest at days: 65 days: 65 25% moistureheat units: 1376.8 heat units: 1286.67 foliage: intensity of greenmedium medium color 4. Plant ratio height of insertion of small mediumupper ear to plant length peduncle length medium very long plant height(to tassel tip) 151.8 cm 137.1 cm standard deviation: standarddeviation: 10.0601 11.3489 sample size: 15 sample size: 18 ear height(to base of top ear 32.9 cm 46.65 cm node) standard deviation: standarddeviation: 6.3128 8.3929 sample size: 15 sample size: 18 length of topear internode 11.3 cm 11.15 cm standard deviation: standard deviation:2.313 1.0291 sample size: 15 sample size: 18 average number of tillers0.8 avg 3.75 avg standard deviation: standard deviation: 0.7432 1.232sample size: 15 sample size: 18 average number of ears per 2 avg 1.8 avgstalk standard deviation: standard deviation: 0.6546 0.25 sample size:15 sample size: 18 anthocyanin of brace roots absent absent 5. Leaffirst leaf: anthocyanin absent or very weak absent or very weakcoloration of sheath first leaf: shape of apex pointed to roundedpointed undulation of margin of blade intermediate intermediate anglebetween blade and stem medium medium (on leaf just above upper ear)curvature of blade moderately recurved moderately recurved anthocyanincoloration of absent of very weak absent of very weak sheath (in middleof plant) width of blade narrow medium width of ear node leaf in 7.3 cm7.8 cm centimeters standard deviation: standard deviation: 0.5462 0.4613sample size: 15 sample size: 18 length of ear node leaf in 68.2 cm 72.7cm centimeters standard deviation: standard deviation: 3.5271 7.6509sample size: 15 sample size: 18 number of leaves above top 7.5 5.45 earstandard deviation: standard deviation: 1.1254 0.6368 sample size: 15sample size: 18 degrees leaf angle 62° 45° color 5GY 3/4 7.5gy 4/4sheath pubescence (1 = none to 2 5 9 = like peach fuzz) marginal waves(1 = none to 4 5 9 = many) longitudinal creases (1 = none 3 1 to 9 =many) stem: degree of zig-zag slight absent or very weak stem:anthocyanin coloration absent or very weak absent or very weak of braceroots stem: anthocyanin coloration absent or very weak absent or veryweak of internodes 6. Tassel time of anthesis (on middle medium late tovery late third of main axis, 50% of plants) anthocyanin coloration atbase absent or very weak absent or very weak of glume (in middle thirdof main axis) anthocyanin coloration of absent or very weak absent orvery weak glumes excluding base anthocyanin coloration of absent or veryweak absent or very weak anthers (in middle third of main axis) anglebetween main axis and medium small lateral branches (in lower third oftassel) curvature of lateral branches moderately recurved slightlyrecurved (in lower third of tassel) number of primary lateral very manymedium branches density of spikelets moderately dense medium length ofmain axis above long medium lowest lateral branch (A-B) length of mainaxis above long short highest lateral branch (C-D) length of lateralbranch short long number of primary lateral 39.8 20.7 branches standarddeviation: standard deviation: 4.3293 2.374 sample size: 15 sample size:18 branch angle from central 42.8° 50.65° spike standard deviation:standard deviation: 7.14 10.2255 sample size: 15 sample size: 18 length(from top leaf collar to 26.5 cm 46.2 cm tassel tip) standard deviation:standard deviation: 1.6198 5.2423 sample size: 15 sample size: 18 pollenshed (0 = male sterile to 5 7 9 = heavy shed) anther color 2.5Y 8/6 5y8/8 glume color 5GY 7/6 5y 7/6 bar glumes (glume bands) absent absent 7.Ear (unhusked data) silk color 2.5GY 8/10 2.5gy 8/10 (3 days afteremergence) (unhusked data) fresh husk 2.5GY 8/6 5gy 7/8 color (25 daysafter 50% silking) (unhusked data) dry husk 5Y 8/4 2.5y 8/4 color (65days after 50% silking) (unhusked data) position of upright upright earat dry husk stage (unhusked data) husk 6 3 tightness (1 = very loose to9 = very tight) (unhusked data) husk long (8-10 cm beyond long extension(at harvest) the ear tip) (husked ear data) ear length 11.3 cm 10.5 cmstandard deviation: standard deviation: 0.9733 1.7386 sample size: 15sample size: 22 (husked ear data) ear diameter 41.5 mm 28.95 mm atmid-point standard deviation: standard deviation: 3.0797 4.3197 samplesize: 15 sample size: 22 (husked ear data) ear weight 47.1 gm 24 gmstandard deviation: standard deviation: 10.9861 8.384 sample size: 15sample size: 22 (husked ear data) number of 18.4 10.1 kernel rowsstandard deviation: standard deviation: 1.2983 2.9065 sample size: 15sample size: 22 (husked ear data) kernel rows distinct distinct (huskedear data) row slightly curved straight alignment (husked ear data) shanklength 12.8 cm 23.4 cm standard deviation: standard deviation: 3.53749.5326 sample size: 15 sample size: 22 (husked ear data) ear taperextreme average length short short diameter (in middle) medium smallshape conical conico-cylindrical number of rows of grain many mediumnumber of colors of grains one one (only varieties with ear type ofgrain: sweet or waxy) grain: intensity of yellow medium medium color(only varieties with ear type of grain: sweet) grain: length (onlyvarieties long medium with ear type of grain: sweet) grain: width (onlyvarieties medium medium with ear type of grain: sweet) type of grainsweet sweet shrinkage of top of grain (only medium strong varieties withear type of grain: sweet) color of top of grain yellow yellowanthocyanin coloration of absent or very weak absent or very weak glumesof cob time of silk emergence (50% medium late to very late of plants)anthocyanin coloration of absent or very weak absent or very weak silks8. Kernel (dried) length 11.1 mm 7 mm standard deviation: standarddeviation: 1.4749 1.0242 sample size: 15 sample size: 30 width 7.3 mm 6mm standard deviation: standard deviation: 0.5011 0.694 sample size: 15sample size: 30 thickness 2.47 mm 5.25 mm standard deviation: standarddeviation: 0.3863 1.191 sample size: 15 sample size: 30 % round kernels(shape grade) 0.00% 1% standard deviation: standard deviation: 0 samplesize: 100 sample size: 200 aleurone color pattern homozygous homozygousaleurone color 2.5Y 8/8 2.5y 8/8 hard endosperm color 2.5Y 8/8 2.5y 8/8endosperm type sweet (su1) sweet weight per 100 kernels 10 gm 12.0 gm(unsized sample) standard deviation: standard deviation: sample size:100 n/a sample size: 200 9. Cob diameter at mid-point 25.5 mm 22.35 mmstandard deviation: standard deviation: 2.4979 2.528 sample size: 15sample size: 22 color 5Y 8/4 2.5y 8/2 12. Agronomic Traits stay green(at 65 days after 3 3 anthesis) (from 1 = worst to 9 = excellent)dropped ears 0.00%   0% % pre-anthesis brittle   0%   0% snapping %pre-anthesis root lodging   0%   0% post-anthesis root lodging 0.00%0.00% *These are typical values. Values may vary due to environment.Other values that are substantially equivalent are also within the scopeof the invention.

TABLE 3 Physiological and Morphological Characteristics of Sweet CornLine SHY 084- 5055 Comparison Variety - Characteristic SHY 084-5055WH92047 1. Type sweet sweet 2. Region where developed in the midwestmidwest U.S.A. 3. Maturity in the Region of Best Adaptability fromemergence to 50% of days: 70 days: 86 plants in silk heat units: 1412.56heat units: 1218.85 from emergence to 50% of days: 69 days: 85 plants inpollen heat units: 1392.52 heat units: 1197.5 from 10% to 90% pollenshed days: 5 days: 3 heat units: 100 heat units: 58 from 50% silk tooptimum days: 26 days: 19 edible quality heat units: 368.97 heat units:396.8 from 50% silk to harvest at days: 62 days: 58 25% moisture heatunits: 842.24 heat units: 1205.9 4. Plant length (tassel included) ShortMedium (inbred lines only) ratio height of insertion of Small mediumupper ear to plant length plant height (to tassel tip) 126.34 cm 91.1 cmstandard deviation: standard deviation: 5.2661 7.4347 sample size: 60sample size: 30 ear height (to base of top ear 27.82 cm 27.95 cm node)standard deviation: standard deviation: 2.0684 2.6191 sample size: 60sample size: 30 length of top ear internode 12.38 cm 12.1 cm standarddeviation: standard deviation: 1.3221 1.1319 sample size: 60 samplesize: 30 average number of tillers 2.48 avg 1.7 avg standard deviation:standard deviation: 1.0441 0.6962 sample size: 60 sample size: 30average number of ears per 1.85 avg 3.3 avg stalk standard deviation:standard deviation: 0.6985 0.5786 sample size: 60 sample size: 30anthocyanin of brace roots absent absent 5. Leaf first leaf: anthocyaninabsent or very weak absent or very weak coloration of sheath first leaf:shape of tip pointed [W117] pointed angle between blade and stem largevery small (on leaf just above upper ear) angle between blade and stemstrongly recurved straight (on leaf just above upper ear) anthocyanincoloration of Absent or very weak Absent or very weak sheath (in middleof plant) width of blade medium [A632] medium width of ear node leaf in4.84 7.57 centimeters standard deviation: standard deviation: 0.30520.5575 sample size: 60 sample size: 30 length of ear node leaf in 7370.6 centimeters standard deviation: standard deviation: 2.2162 3.2304sample size: 60 Sample size: 30 number of leaves above top 5.27 7.5 earstandard deviation: standard deviation: 0.6295 1.1313 sample size: 60sample size: 30 degrees leaf angle 42.3 37 color 5gy 4/4 5gy 4/4 sheathpubescence (1 = none to 2 5 9 = like peach fuzz) marginal waves (1 =none to 3 6 9 = many) longitudinal creases (1 = none 1 1 to 9 = many)stem: degree of zig-zag absent or very slight absent or very slightstem: anthocyanin coloration absent or very weak weak of brace roots 6.Tassel time of anthesis (on middle very early to early medium third ofmain axis, 50% of plants) anthocyanin coloration at base absent or veryweak absent or very weak of glume (in middle third of main axis)anthocyanin coloration of medium absent or very weak glumes excludingbase anthocyanin coloration of weak absent or very weak anthers (inmiddle third of main axis) angle between main axis and very small mediumlateral branches (in lower third of tassel) attitude of lateral branches(in slightly recurved straight lower third of tassel) number of primarylateral 25.27 13.93 branches standard deviation: standard deviation:4.3424 3.0533 sample size: 60 sample size: 30 density of spikeletsmedium [W401] lax length of main axis above short [EP1] short lowestside branch length of main axis above medium [F259] medium upper sidebranch length of side branches short [f2] medium number of primarylateral medium [F244] medium branches branch angle from central 40.1°44° spike standard deviation: standard deviation: 9.5581 13.7209 samplesize: 60 sample size: 30 length (from top leaf collar to 38.97 cm 24 cmtassel tip) standard deviation: standard deviation: 2.3796 1.5257 samplesize: 60 sample size: 30 pollen shed (0 = male sterile to 7 6 9 = heavyshed) anther color 2.5gy 7/6 2.5gy 7/6 glume color 5gy 7/6 5gy 6/6 barglumes (glume bands) Absent absent 7. Ear (unhusked data) silk color2.5gy 8/6 2.5gy 8/8 (3 days after emergence) (unhusked data) fresh husk5gy 5/6 2.5gy 6/6 color (25 days after 50% silking) (unhusked data) dryhusk 2.5gy 7/6 2.5y 8/6 color (65 days after 50% silking) (unhuskeddata) position of upright upright ear at dry husk stage (unhusked data)husk 8 6 tightness (1 = very loose to 9 = very tight) (unhusked data)husk Long (8-10 cm beyond medium extension (at harvest) the . . . )(husked ear data) ear length 14.29 10.55 in centimeters standarddeviation: standard deviation: 2.0651 1.4513 sample size: 60 samplesize: 30 (husked ear data) ear diameter 28.74 34.49avg at mid-point inmillimeters standard deviation: standard deviation: 8.5982 3.8469 samplesize: 60 sample size: 30 (husked ear data) ear weight 24.15 36.67 ingrams standard deviation: standard deviation: 9.8383 12.3794 samplesize: 60 sample size: 30 (husked ear data) number of 14 15.13 kernelrows standard deviation: standard deviation: 2.6167 2.6821 sample size:60 sample size: 30 (husked ear data) kernel rows Indistinct distinct(husked ear data) row slightly curved straight alignment (husked eardata) shank length 8.77 8.98 in centimeters standard deviation: standarddeviation: 3.0986 2.9215 sample size: 60 sample size: 30 (husked eardata) ear taper extreme slight length of peduncle medium [W182E] shortlength (without husk) medium [A65] short diameter (in middle) Mediumsmall shape Cylindrical Conico-cylindrical number of rows of grain Fewmedium color of dorsal side of grain yellow [A654] yellow type of grainsweet [Jubilee] sweet color of top of grain Yellow orange Yellow orangeanthocyanin coloration of Absent Absent glumes of cob time of silkemergence (50% Very late Late to very late of plants) anthocyanincoloration of Absent Absent silks 8. Kernel (dried) Length (mm) 6.857.27 standard deviation: standard deviation: 1.1008 1.2408 sample size:60 sample size: 30 Width (mm) 6.84 7 standard deviation: standarddeviation: 0.9221 0.833 sample size: 60 sample size: 30 Thickness (mm)4.23 4.87 standard deviation: standard deviation: 1.0895 1.3291 samplesize: 60 sample size: 30 % round kernels (shape grade) 49.75 2.00standard deviation 0 standard deviation: 0 sample size: 60 sample size:30 aleurone color pattern 1 1 aleurone color 2.5y 8/10 2.5y 8/10 hardendosperm color 2.5y 8/10 2.5y 8/10 endosperm type sweet (su1) sweetweight per 100 kernels 8.23 13.5 (unsized sample) standard deviation: 0standard deviation: 0 sample size: 200 sample size: 100 9. Cob diameterat mid-point 23.81 22.56 standard deviation: standard deviation: 2.20612.1828 sample size: 60 sample size: 30 color 5y 8/4 5y 8/4 12. AgronomicTraits dropped ears 0% 0% % pre-anthesis brittle 0% 0% snapping %pre-anthesis root lodging 0% 0% post-anthesis root lodging 0% 0% *Theseare typical values. Values may vary due to environment. Other valuesthat are substantially equivalent are also within the scope of theinvention.

C. BREEDING CORN PLANTS

One aspect of the current invention concerns methods for producing seedof sweet corn hybrid SHY6RH1365 involving crossing sweet corn linesSHY-6RLAL201 and SHY 084-5055. Alternatively, in other embodiments ofthe invention, hybrid SHY6RH1365, line SHY-6RLAL201, or line SHY084-5055 may be crossed with itself or with any second plant. Suchmethods can be used for propagation of hybrid SHY6RH1365 and/or thesweet corn lines SHY-6RLAL201 and SHY 084-5055, or can be used toproduce plants that are derived from hybrid SHY6RH1365 and/or the sweetcorn lines SHY-6RLAL201 and SHY 084-5055. Plants derived from hybridSHY6RH1365 and/or the sweet corn lines SHY-6RLAL201 and SHY 084-5055 maybe used, in certain embodiments, for the development of new cornvarieties.

The development of new varieties using one or more starting varieties iswell known in the art. In accordance with the invention, novel varietiesmay be created by crossing hybrid SHY6RH1365 followed by multiplegenerations of breeding according to such well known methods. Newvarieties may be created by crossing with any second plant. In selectingsuch a second plant to cross for the purpose of developing novel lines,it may be desired to choose those plants which either themselves exhibitone or more selected desirable characteristics or which exhibit thedesired characteristic(s) when in hybrid combination. Once initialcrosses have been made, inbreeding and selection take place to producenew varieties. For development of a uniform line, often five or moregenerations of selfing and selection are involved.

Uniform lines of new varieties may also be developed by way ofdouble-haploids. This technique allows the creation of true breedinglines without the need for multiple generations of selfing andselection. In this manner true breeding lines can be produced in aslittle as one generation. Haploid embryos may be produced frommicrospores, pollen, anther cultures, or ovary cultures. The haploidembryos may then be doubled autonomously, or by chemical treatments(e.g. colchicine treatment). Alternatively, haploid embryos may be growninto haploid plants and treated to induce chromosome doubling. In eithercase, fertile homozygous plants are obtained. In accordance with theinvention, any of such techniques may be used in connection with a plantof the invention and progeny thereof to achieve a homozygous line.

Backcrossing can also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or non-inbredsource to an inbred that lacks that trait. This can be accomplished, forexample, by first crossing a superior inbred (A) (recurrent parent) to adonor inbred (non-recurrent parent), which carries the appropriate locusor loci for the trait in question. The progeny of this cross are thenmated back to the superior recurrent parent (A) followed by selection inthe resultant progeny for the desired trait to be transferred from thenon-recurrent parent. After five or more backcross generations withselection for the desired trait, the progeny have the characteristicbeing transferred, but are like the superior parent for most or almostall other loci. The last backcross generation would be selfed to givepure breeding progeny for the trait being transferred.

The plants of the present invention are particularly well suited for thedevelopment of new lines based on the elite nature of the geneticbackground of the plants. In selecting a second plant to cross withSHY6RH1365 and/or sweet corn lines SHY-6RLAL201 and SHY 084-5055 for thepurpose of developing novel corn lines, it will typically be preferredto choose those plants which either themselves exhibit one or moreselected desirable characteristics or which exhibit the desiredcharacteristic(s) when in hybrid combination. Examples of desirabletraits may include, in specific embodiments, male sterility, herbicideresistance, resistance for bacterial, fungal, or viral disease, insectresistance, male fertility, sugar content, and enhanced nutritionalquality.

D. PERFORMANCE CHARACTERISTICS

As described above, hybrid SHY6RH1365 exhibits desirable agronomictraits. The performance characteristics of hybrid SHY6RH1365 were thesubject of an objective analysis of the performance traits relative toother varieties. The results of the analysis are presented below.

TABLE 4 Performance Data for Hybrid SHY6RH1365 and Comparative VarietyHEAT PERCENT OF EARS WITH UNITS EAR A ROW COUNT OF. TO PLANT EAR EARDIAM- Greater Planting MID- HEIGHT HEIGHT LENGTH ETER than Date HYBRIDRep# SILK (inches) (inches) (inches (inches) 12 14 16 18 20 20 May 20,2011 SHY6RH1365 1 1239 79 25 8.4 1.9 0 0 30 40 30 0 May 20, 2011SHY6RH1365 2 1239 76 25 8.6 1.9 0 10 40 50 0 0 May 20, 2011 SHY6RH1365 31239 79 35 8.5 1.95 0 0 20 30 50 0 AVERAGE 1239.0 78.0 28.3 8.5 1.9 0.03.3 30.0 40.0 26.7 0.0 May 20, 2011 BASIN R 1 1267 81 37 9.3 1.7 0 60 400 0 0 May 20, 2011 BASIN R 2 1267 74 33 9.2 1.7 10 60 30 0 0 0 May 20,2011 BASIN R 3 1239 82 26 9.3 1.6 0 50 30 20 0 0 AVERAGE 1257.7 79.032.0 9.3 1.7 3.3 56.7 33.3 6.7 0.0 0.0 Jun. 8, 2011 SHY6RH1365 1 1193 9134 8.2 2 0 0 20 50 30 0 Jun. 8, 2011 SHY6RH1365 2 1193 83 30 8.4 1.9 0 030 40 30 0 Jun. 8, 2011 SHY6RH1365 3 1164 86 28 8.3 1.9 0 0 30 60 10 0AVERAGE 1183.3 86.7 30.7 8.3 1.9 0.0 0.0 26.7 50.0 23.3 0.0 Jun. 8, 2011BASIN R 1 1255 91 28 9.4 1.8 0 30 60 10 0 0 Jun. 8, 2011 BASIN R 2 116488 27 9.1 1.9 0 20 70 10 0 0 Jun. 8, 2011 BASIN R 3 1193 85 26 9.2 1.710 0 50 40 0 0 AVERAGE 1204.0 88.0 27.0 9.2 1.8 3.3 16.7 60.0 20.0 0.00.0 SHY6RH1365 TOTAL 1211.2 82.3 29.5 8.4 1.9 0.0 1.7 28.3 45.0 25.0 0.0AVG BASIN R TOTAL 1230.8 83.5 29.5 9.3 1.7 3.3 36.7 46.7 13.3 0.0 0.0AVG

E. FURTHER EMBODIMENTS OF THE INVENTION

In certain aspects of the invention, plants described herein areprovided modified to include at least a first desired heritable trait.Such plants may, in one embodiment, be developed by a plant breedingtechnique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to a genetic locus transferred into the plant viathe backcrossing technique. The term single locus converted plant asused herein refers to those corn plants which are developed by a plantbreeding technique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to the single locus transferred into the varietyvia the backcrossing technique. By essentially all of the morphologicaland physiological characteristics, it is meant that the characteristicsof a plant are recovered that are otherwise present when compared in thesame environment, other than an occasional variant trait that mightarise during backcrossing or direct introduction of a transgene.

Backcrossing methods can be used with the present invention to improveor introduce a characteristic into the present variety. The parentalcorn plant which contributes the locus for the desired characteristic istermed the nonrecurrent or donor parent. This terminology refers to thefact that the nonrecurrent parent is used one time in the backcrossprotocol and therefore does not recur. The parental corn plant to whichthe locus or loci from the nonrecurrent parent are transferred is knownas the recurrent parent as it is used for several rounds in thebackcrossing protocol.

In a typical backcross protocol, the original variety of interest(recurrent parent) is crossed to a second variety (nonrecurrent parent)that carries the single locus of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a corn plant isobtained wherein essentially all of the morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant, in addition to the single transferred locus from the nonrecurrentparent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single locus of the recurrent variety ismodified or substituted with the desired locus from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphologicalconstitution of the original variety. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross; one ofthe major purposes is to add some commercially desirable trait to theplant. The exact backcrossing protocol will depend on the characteristicor trait being altered and the genetic distance between the recurrentand nonrecurrent parents. Although backcrossing methods are simplifiedwhen the characteristic being transferred is a dominant allele, arecessive allele, or an additive allele (between recessive anddominant), may also be transferred. In this instance it may be necessaryto introduce a test of the progeny to determine if the desiredcharacteristic has been successfully transferred.

In one embodiment, progeny corn plants of a backcross in which a plantdescribed herein is the recurrent parent comprise (i) the desired traitfrom the non-recurrent parent and (ii) all of the physiological andmorphological characteristics of corn the recurrent parent as determinedat the 5% significance level when grown in the same environmentalconditions.

New varieties can also be developed from more than two parents. Thetechnique, known as modified backcrossing, uses different recurrentparents during the backcrossing. Modified backcrossing may be used toreplace the original recurrent parent with a variety having certain moredesirable characteristics or multiple parents may be used to obtaindifferent desirable characteristics from each.

With the development of molecular markers associated with particulartraits, it is possible to add additional traits into an established germline, such as represented here, with the end result being substantiallythe same base germplasm with the addition of a new trait or traits.Molecular breeding, as described in Moose and Mumm, 2008 (PlantPhysiology, 147: 969-977), for example, and elsewhere, provides amechanism for integrating single or multiple traits or QTL into an eliteline. This molecular breeding-facilitated movement of a trait or traitsinto an elite line may encompass incorporation of a particular genomicfragment associated with a particular trait of interest into the eliteline by the mechanism of identification of the integrated genomicfragment with the use of flanking or associated marker assays. In theembodiment represented here, one, two, three or four genomic loci, forexample, may be integrated into an elite line via this methodology. Whenthis elite line containing the additional loci is further crossed withanother parental elite line to produce hybrid offspring, it is possibleto then incorporate at least eight separate additional loci into thehybrid. These additional loci may confer, for example, such traits as adisease resistance or a fruit quality trait. In one embodiment, eachlocus may confer a separate trait. In another embodiment, loci may needto be homozygous and exist in each parent line to confer a trait in thehybrid. In yet another embodiment, multiple loci may be combined toconfer a single robust phenotype of a desired trait.

Many single locus traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single locus traits may or may not betransgenic; examples of these traits include, but are not limited to,male sterility, waxy starch, herbicide resistance, resistance forbacterial, fungal, or viral disease, insect resistance, sugar content,male fertility and enhanced nutritional quality. These genes aregenerally inherited through the nucleus, but may be inherited throughthe cytoplasm. Some known exceptions to this are genes for malesterility, some of which are inherited cytoplasmically, but still act asa single locus trait.

Direct selection may be applied where the single locus acts as adominant trait. For this selection process, the progeny of the initialcross are assayed for viral resistance and/or the presence of thecorresponding gene prior to the backcrossing. Selection eliminates anyplants that do not have the desired gene and resistance trait, and onlythose plants that have the trait are used in the subsequent backcross.This process is then repeated for all additional backcross generations.

Selection of corn plants for breeding is not necessarily dependent onthe phenotype of a plant and instead can be based on geneticinvestigations. For example, one can utilize a suitable genetic markerwhich is closely genetically linked to a trait of interest. One of thesemarkers can be used to identify the presence or absence of a trait inthe offspring of a particular cross, and can be used in selection ofprogeny for continued breeding. This technique is commonly referred toas marker assisted selection. Any other type of genetic marker or otherassay which is able to identify the relative presence or absence of atrait of interest in a plant can also be useful for breeding purposes.Procedures for marker assisted selection are well known in the art. Suchmethods will be of particular utility in the case of recessive traitsand variable phenotypes, or where conventional assays may be moreexpensive, time consuming or otherwise disadvantageous. Types of geneticmarkers which could be used in accordance with the invention include,but are not necessarily limited to, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990),Randomly Amplified Polymorphic DNAs (RAPDs), DNA AmplificationFingerprinting (DAF), Sequence Characterized Amplified Regions (SCARs),Arbitrary Primed Polymerase Chain Reaction (AP-PCR), Amplified FragmentLength Polymorphisms (AFLPs) (EP 534 858, specifically incorporatedherein by reference in its entirety), and Single NucleotidePolymorphisms (SNPs) (Wang et al., Science, 280:1077-1082, 1998).

F. PLANTS DERIVED BY GENETIC ENGINEERING

Many useful traits that can be introduced by backcrossing, as well asdirectly into a plant, are those which are introduced by genetictransformation techniques. Genetic transformation may therefore be usedto insert a selected transgene into a plant of the invention or may,alternatively, be used for the preparation of transgenes which can beintroduced by backcrossing. Methods for the transformation of plantsthat are well known to those of skill in the art and applicable to manycrop species include, but are not limited to, electroporation,microprojectile bombardment, Agrobacterium-mediated transformation anddirect DNA uptake by protoplasts.

To effect transformation by electroporation, one may employ eitherfriable tissues, such as a suspension culture of cells or embryogeniccallus or alternatively one may transform immature embryos or otherorganized tissue directly. In this technique, one would partiallydegrade the cell walls of the chosen cells by exposing them topectin-degrading enzymes (pectolyases) or mechanically wound tissues ina controlled manner.

An efficient method for delivering transforming DNA segments to plantcells is microprojectile bombardment. In this method, particles arecoated with nucleic acids and delivered into cells by a propellingforce. Exemplary particles include those comprised of tungsten,platinum, and preferably, gold. For the bombardment, cells in suspensionare concentrated on filters or solid culture medium. Alternatively,immature embryos or other target cells may be arranged on solid culturemedium. The cells to be bombarded are positioned at an appropriatedistance below the macroprojectile stopping plate.

An illustrative embodiment of a method for delivering DNA into plantcells by acceleration is the Biolistics Particle Delivery System, whichcan be used to propel particles coated with DNA or cells through ascreen, such as a stainless steel or Nytex screen, onto a surfacecovered with target cells. The screen disperses the particles so thatthey are not delivered to the recipient cells in large aggregates.Microprojectile bombardment techniques are widely applicable, and may beused to transform virtually any plant species.

Agrobacterium-mediated transfer is another widely applicable system forintroducing gene loci into plant cells. An advantage of the technique isthat DNA can be introduced into whole plant tissues, thereby bypassingthe need for regeneration of an intact plant from a protoplast. ModernAgrobacterium transformation vectors are capable of replication in E.coli as well as Agrobacterium, allowing for convenient manipulations(Klee et al., Bio-Technology, 3(7):637-642, 1985). Moreover, recenttechnological advances in vectors for Agrobacterium-mediated genetransfer have improved the arrangement of genes and restriction sites inthe vectors to facilitate the construction of vectors capable ofexpressing various polypeptide coding genes. The vectors described haveconvenient multi-linker regions flanked by a promoter and apolyadenylation site for direct expression of inserted polypeptidecoding genes. Additionally, Agrobacterium containing both armed anddisarmed Ti genes can be used for transformation.

In those plant strains where Agrobacterium-mediated transformation isefficient, it is the method of choice because of the facile and definednature of the gene locus transfer. The use of Agrobacterium-mediatedplant integrating vectors to introduce DNA into plant cells is wellknown in the art (Fraley et al., Bio/Technology, 3:629-635, 1985; U.S.Pat. No. 5,563,055).

Transformation of plant protoplasts also can be achieved using methodsbased on calcium phosphate precipitation, polyethylene glycol treatment,electroporation, and combinations of these treatments (see, e.g.,Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985; Omirulleh et al.,Plant Mol. Biol., 21(3):415-428, 1993; Fromm et al., Nature,312:791-793, 1986; Uchimiya et al., Mol. Gen. Genet., 204:204, 1986;Marcotte et al., Nature, 335:454, 1988). Transformation of plants andexpression of foreign genetic elements is exemplified in Choi et al.(Plant Cell Rep., 13: 344-348, 1994), and Ellul et al. (Theor. Appl.Genet., 107:462-469, 2003).

A number of promoters have utility for plant gene expression for anygene of interest including but not limited to selectable markers,scoreable markers, genes for pest tolerance, disease resistance,nutritional enhancements and any other gene of agronomic interest.Examples of constitutive promoters useful for plant gene expressioninclude, but are not limited to, the cauliflower mosaic virus (CaMV)P-35S promoter, which confers constitutive, high-level expression inmost plant tissues (see, e.g., Odel et al., Nature, 313:810, 1985),including in monocots (see, e.g., Dekeyser et al., Plant Cell, 2:591,1990; Terada and Shimamoto, Mol. Gen. Genet., 220:389, 1990); a tandemlyduplicated version of the CaMV 35S promoter, the enhanced 35S promoter(P-e35S); 1 the nopaline synthase promoter (An et al., Plant Physiol.,88:547, 1988); the octopine synthase promoter (Fromm et al., Plant Cell,1:977, 1989); and the figwort mosaic virus (P-FMV) promoter as describedin U.S. Pat. No. 5,378,619 and an enhanced version of the FMV promoter(P-eFMV) where the promoter sequence of P-FMV is duplicated in tandem;the cauliflower mosaic virus 19S promoter; a sugarcane bacilliform viruspromoter; a commelina yellow mottle virus promoter; and other plant DNAvirus promoters known to express in plant cells.

A variety of plant gene promoters that are regulated in response toenvironmental, hormonal, chemical, and/or developmental signals can alsobe used for expression of an operably linked gene in plant cells,including promoters regulated by (1) heat (Callis et al., PlantPhysiol., 88:965, 1988), (2) light (e.g., pea rbcS-3A promoter,Kuhlemeier et al., Plant Cell, 1:471, 1989; maize rbcS promoter,Schaffner and Sheen, Plant Cell, 3:997, 1991; or chlorophyll a/b-bindingprotein promoter, Simpson et al., EMBO J., 4:2723, 1985), (3) hormones,such as abscisic acid (Marcotte et al., Plant Cell, 1:969, 1989), (4)wounding (e.g., wunl, Siebertz et al., Plant Cell, 1:961, 1989); or (5)chemicals such as methyl jasmonate, salicylic acid, or Safener. It mayalso be advantageous to employ organ-specific promoters (e.g., Roshal etal., EMBO J., 6:1155, 1987; Schernthaner et al., EMBO J., 7:1249, 1988;Bustos et al., Plant Cell, 1:839, 1989).

Exemplary nucleic acids which may be introduced to plants of thisinvention include, for example, DNA sequences or genes from anotherspecies, or even genes or sequences which originate with or are presentin the same species, but are incorporated into recipient cells bygenetic engineering methods rather than classical reproduction orbreeding techniques. However, the term “exogenous” is also intended torefer to genes that are not normally present in the cell beingtransformed, or perhaps simply not present in the form, structure, etc.,as found in the transforming DNA segment or gene, or genes which arenormally present and that one desires to express in a manner thatdiffers from the natural expression pattern, e.g., to over-express.Thus, the term “exogenous” gene or DNA is intended to refer to any geneor DNA segment that is introduced into a recipient cell, regardless ofwhether a similar gene may already be present in such a cell. The typeof DNA included in the exogenous DNA can include DNA which is alreadypresent in the plant cell, DNA from another plant, DNA from a differentorganism, or a DNA generated externally, such as a DNA sequencecontaining an antisense message of a gene, or a DNA sequence encoding asynthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and couldpotentially be introduced into a corn plant according to the invention.Non-limiting examples of particular genes and corresponding phenotypesone may choose to introduce into a corn plant include one or more genesfor insect tolerance, such as a Bacillus thuringiensis (B.t.) gene, pesttolerance such as genes for fungal disease control, herbicide tolerancesuch as genes conferring glyphosate tolerance, and genes for qualityimprovements such as yield, nutritional enhancements, environmental orstress tolerances, or any desirable changes in plant physiology, growth,development, morphology or plant product(s). For example, structuralgenes would include any gene that confers insect tolerance including butnot limited to a Bacillus insect control protein gene as described in WO99/31248, herein incorporated by reference in its entirety, U.S. Pat.No. 5,689,052, herein incorporated by reference in its entirety, U.S.Pat. Nos. 5,500,365 and 5,880,275, herein incorporated by reference intheir entirety. In another embodiment, the structural gene can confertolerance to the herbicide glyphosate as conferred by genes including,but not limited to Agrobacterium strain CP4 glyphosate resistant EPSPSgene (aroA:CP4) as described in U.S. Pat. No. 5,633,435, hereinincorporated by reference in its entirety, or glyphosate oxidoreductasegene (GOX) as described in U.S. Pat. No. 5,463,175, herein incorporatedby reference in its entirety.

Alternatively, the DNA coding sequences can affect these phenotypes byencoding a non-translatable RNA molecule that causes the targetedinhibition of expression of an endogenous gene, for example viaantisense- or cosuppression-mediated mechanisms (see, for example, Birdet al., Biotech. Gen. Engin. Rev., 9:207, 1991). The RNA could also be acatalytic RNA molecule (i.e., a ribozyme) engineered to cleave a desiredendogenous mRNA product (see for example, Gibson and Shillito, Mol.Biotech., 7:125, 1997). Thus, any gene which produces a protein or mRNAwhich expresses a phenotype or morphology change of interest is usefulfor the practice of the present invention.

G. DEFINITIONS

In the description and tables herein, a number of terms are used. Inorder to provide a clear and consistent understanding of thespecification and claims, the following definitions are provided:

Allele: Any of one or more alternative forms of a gene locus, all ofwhich alleles relate to one trait or characteristic. In a diploid cellor organism, the two alleles of a given gene occupy corresponding locion a pair of homologous chromosomes.

Backcrossing: A process in which a breeder repeatedly crosses hybridprogeny, for example a first generation hybrid (F₁), back to one of theparents of the hybrid progeny. Backcrossing can be used to introduce oneor more single locus conversions from one genetic background intoanother.

Crossing: The mating of two parent plants.

Cross-pollination: Fertilization by the union of two gametes fromdifferent plants.

Diploid: A cell or organism having two sets of chromosomes.

Emasculate: The removal of plant male sex organs or the inactivation ofthe organs with a cytoplasmic or nuclear genetic factor or a chemicalagent conferring male sterility.

Enzymes: Molecules which can act as catalysts in biological reactions.

F₁ Hybrid: The first generation progeny of the cross of two nonisogenicplants.

Genotype: The genetic constitution of a cell or organism.

Haploid: A cell or organism having one set of the two sets ofchromosomes in a diploid.

Linkage: A phenomenon wherein alleles on the same chromosome tend tosegregate together more often than expected by chance if theirtransmission was independent.

Marker: A readily detectable phenotype, preferably inherited incodominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,heritability of 1.

Phenotype: The detectable characteristics of a cell or organism, whichcharacteristics are the manifestation of gene expression.

Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer togenetic loci that control to some degree numerically representabletraits that are usually continuously distributed.

Resistance: As used herein, the terms “resistance” and “tolerance” areused interchangeably to describe plants that show no symptoms to aspecified biotic pest, pathogen, abiotic influence or environmentalcondition. These terms are also used to describe plants showing somesymptoms but that are still able to produce marketable product with anacceptable yield. Some plants that are referred to as resistant ortolerant are only so in the sense that they may still produce a crop,even though the plants are stunted and the yield is reduced.

Regeneration: The development of a plant from tissue culture.

Self-pollination: The transfer of pollen from the anther to the stigmaof the same plant.

Single Locus Converted (Conversion) Plant: Plants which are developed bya plant breeding technique called backcrossing, wherein essentially allof the morphological and physiological characteristics of a corn varietyare recovered in addition to the characteristics of the single locustransferred into the variety via the backcrossing technique and/or bygenetic transformation.

Substantially Equivalent: A characteristic that, when compared, does notshow a statistically significant difference (e.g., p=0.05) from themean.

Tissue Culture: A composition comprising isolated cells of the same or adifferent type or a collection of such cells organized into parts of aplant.

Transgene: A genetic locus comprising a sequence which has beenintroduced into the genome of a corn plant by transformation.

H. DEPOSIT INFORMATION

A deposit of sweet corn hybrid SHY6RH1365 and parent lines SHY-6RLAL201and SHY 084-5055, disclosed above and recited in the claims, has beenmade with the American Type Culture Collection (ATCC), 10801 UniversityBlvd., Manassas, Va. 20110-2209. The date of the deposits were, May 31,2012, May 15, 2012, and May 15, 2012, respectively. The accessionnumbers for those deposited seeds of sweet corn hybrid SHY6RH1365 andparent line SHY 084-5055 are ATCC Accession Number PTA-12926, ATCCAccession Number PTA-12892, and ATCC Accession Number PTA-12894,respectively. Upon issuance of a patent, all restrictions upon thedeposits will be removed, and the deposits are intended to meet all ofthe requirements of 37 C.F.R. §1.801-1.809. The deposits will bemaintained in the depository for a period of 30 years, or 5 years afterthe last request, or for the effective life of the patent, whichever islonger, and will be replaced if necessary during that period.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

All references cited herein are hereby expressly incorporated herein byreference.

What is claimed is:
 1. A corn plant comprising at least a first set ofthe chromosomes of corn line SHY 084-5055, a sample of seed of said linehaving been deposited under ATCC Accession Number PTA-12894.
 2. A seedcomprising at least a first set of the chromosomes of corn line SHY084-5055, a sample of seed of said line having been deposited under ATCCAccession Number PTA-12894.
 3. The plant of claim 1, which is an inbred.4. The plant of claim 1, which is a hybrid.
 5. The seed of claim 2,which is an inbred.
 6. The seed of claim 2, which is a hybrid.
 7. Theplant of claim 4, wherein the hybrid plant is corn hybrid SHY6RH1365, asample of seed of said hybrid SHY6RH1365 having been deposited underATCC Accession Number PTA-12926.
 8. The seed of claim 6, defined as aseed of corn hybrid SHY6RH1365, a sample of seed of said hybridSHY6RH1365 having been deposited under ATCC Accession Number PTA-12926.9. The seed of claim 2, defined as a seed of line SHY 084-5055.
 10. Aplant part of the plant of claim
 1. 11. The plant part of claim 10,further defined as an ear, ovule, pollen or cell.
 12. A corn planthaving all the physiological and morphological characteristics of thecorn plant of claim
 7. 13. A tissue culture of regenerable cells of theplant of claim
 1. 14. The tissue culture according to claim 13,comprising cells or protoplasts from a plant part selected from thegroup consisting of leaf, pollen, embryo, root, root tip, anther, silk,flower, kernel, ear, cob, husk, stalk and meristem.
 15. A corn plantregenerated from the tissue culture of claim
 13. 16. A method ofvegetatively propagating the plant of claim 1 comprising the steps of:(a) collecting tissue capable of being propagated from a plant accordingto claim 1; (b) cultivating said tissue to obtain proliferated shoots;and (c) rooting said proliferated shoots to obtain rooted plantlets. 17.The method of claim 16, further comprising growing at least a firstplant from said rooted plantlets.
 18. A method of introducing a desiredtrait into a corn line comprising: (a) crossing a plant of line SHY084-5055 with a second corn plant that comprises a desired trait toproduce F1 progeny, a sample of seed of said line having been depositedunder ATCC Accession Number PTA-12894; (b) selecting an F1 progeny thatcomprises the desired trait; (c) backcrossing the selected F1 progenywith a plant of line SHY 084-5055 to produce backcross progeny; (d)selecting backcross progeny comprising the desired trait and thephysiological and morphological characteristic of corn line SHY084-5055; and (e) repeating steps (c) and (d) three or more times toproduce selected fourth or higher backcross progeny that comprise thedesired trait.
 19. A corn plant produced by the method of claim
 18. 20.A method of producing a plant comprising an added trait, the methodcomprising introducing a transgene conferring the trait into a plant ofhybrid SHY6RH1365, or line SHY 084-5055, a sample of seed of said hybridand line having been deposited under ATCC Accession Number PTA-12926,and ATCC Accession Number PTA-12894, respectively.
 21. A plant producedby the method of claim
 20. 22. The plant of claim 1, further comprisinga transgene.
 23. The plant of claim 22, wherein the transgene confers atrait selected from the group consisting of male sterility, herbicidetolerance, insect resistance, pest resistance, disease resistance,modified fatty acid metabolism, environmental stress tolerance, modifiedcarbohydrate metabolism and modified protein metabolism.
 24. The plantof claim 1, further comprising a single locus conversion.
 25. The plantof claim 24, wherein the single locus conversion confers a traitselected from the group consisting of male sterility, herbicidetolerance, insect resistance, pest resistance, disease resistance,modified fatty acid metabolism, environmental stress tolerance, modifiedcarbohydrate metabolism and modified protein metabolism.
 26. A methodfor producing a seed of a plant derived from at least one of hybridSHY6RH1365, or line SHY 084-5055 comprising the steps of: (a) crossing acorn plant of hybrid SHY6RH1365, or line SHY 084-5055 with itself or asecond corn plant; a sample of seed of said hybrid and line having beendeposited under ATCC Accession Number PTA-12926, and ATCC AccessionNumber PTA-12894, respectively; and (b) allowing seed of a hybridSHY6RH1365, or line SHY 084-5055-derived corn plant to form.
 27. Themethod of claim 26, further comprising the steps of: (c) selfing a plantgrown from said hybrid SHY6RH1365, or SHY 084-5055-derived corn seed toyield additional hybrid SHY6RH1365, or line SHY 084-5055-derived cornseed; (d) growing said additional hybrid SHY6RH1365, or line SHY084-5055-derived corn seed of step (c) to yield additional hybridSHY6RH1365, or line SHY 084-5055-derived corn plants; and (e) repeatingthe crossing and growing steps of (c) and (d) to generate at least afirst further hybrid SHY6RH1365, or line SHY 084-5055-derived cornplant.
 28. The method of claim 26, wherein the second corn plant is ofan inbred corn line.
 29. The method of claim 26, comprising crossingline SHY 084-5055 with line SHY-6RLAL201, a sample of seed of said lineshaving been deposited under ATCC Accession Number PTA-12894, and ATCCAccession Number PTA-12892, respectively.
 30. The method of claim 27,further comprising: (f) crossing the further hybrid SHY6RH1365, or SHY084-5055-derived corn plant with a second corn plant to produce seed ofa hybrid progeny plant.
 31. A plant part of the plant of claim
 7. 32.The plant part of claim 31, further defined as a fruit, a ovule, pollen,a leaf, or a cell.
 33. A method of producing a corn seed comprisingcrossing the plant of claim 1 with itself or a second corn plant andallowing seed to form.
 34. A method of producing a corn comprising: (a)obtaining a plant according to claim 1, wherein the plant has beencultivated to maturity; and (b) collecting a corn from the plant.
 35. Amethod of producing a plant of corn hybrid SHY6RH1365 comprising asingle locus conversion, the method comprising crossing a plant of lineSHY-6RLAL201 with a plant of line SHY 084-5055, wherein at least one ofsaid lines comprises a single locus conversion, a sample of seed of saidlines SHY-6RLAL201 and SHY 084-5055 having been deposited under ATCCAccession No. PTA-12892 and ATCC Accession Number PTA-12894,respectively.